Semi Prep HPLC Column, NP Amino, Wide Pore, 10um, 300A. 10mm ID x 250mm Length. Cogent Brand. 1 EA.
Additional Information:
Cogent NP Amino Wide Pore is a versatile normal phase and HILIC stationary phase designed for retention of highly polar compounds and larger molecules that are often difficult to retain using conventional reversed-phase columns. Manufactured on high-purity 300 Å wide pore silica with a propyl amino bonded phase, it provides excellent selectivity for complex carbohydrates, sugars, organic acids, and other highly polar analytes. This stationary phase is commonly used as an alternative for USP L8 methods and is suitable for both Normal Phase and HILIC separations involving larger molecular species.
68040-SP250-3M is a semi-preparative HPLC column packed with 10 µm NP Amino Wide Pore particles featuring a 300 Å pore size in a 10 mm ID x 250 mm format. The larger internal diameter provides increased sample loading capacity compared to analytical columns while maintaining efficient chromatographic performance. The 250 mm length maximizes resolving power and peak capacity for demanding purification applications. The wide pore structure improves accessibility for larger molecules. Typical operating flow rates are approximately 8–15 mL/min depending on mobile phase composition, sample load, and system configuration.
This column is particularly effective for complex sugars, carbohydrates, negatively charged compounds, organic acids, carboxylic acid-containing compounds, and other highly polar molecules. The wide pore design provides improved performance for larger analytes in both Normal Phase and HILIC applications. As with all amino phases, this column is suitable for HILIC applications when enhanced retention of polar compounds is required.
Key Benefits
NP Amino Wide Pore stationary phase
USP L8 equivalent chemistry
Propyl amino bonded phase
300 Å wide pore silica
Suitable for Normal Phase chromatography
Suitable for HILIC applications
Enhanced retention of polar compounds
Improved accessibility for larger molecules
Low operating backpressure with 10 µm particles
Maximum resolving power for semi-preparative purification
Typical Applications
Semi-preparative chromatography
USP L8 methods
HILIC separations
Normal Phase chromatography
Complex sugar purification
Carbohydrate purification
Organic acid analysis and purification
Carboxylic acid separations
Pharmaceutical purification
Method scale-up from analytical columns
Specifications
Product Type: Semi Prep HPLC Column
Stationary Phase: NP Amino Wide Pore
USP Classification: L8
Chromatography Mode: Normal Phase / HILIC
Bonded Phase: Propyl Amino
Endcapping: Not Endcapped
Particle Size: 10 µm
Pore Size: 300 Å
Column Dimensions: 10 mm ID x 250 mm
Length: 250 mm
Typical Flow Rate: 8–15 mL/min
Brand: Cogent
Special Order Item Information
Important Notes
Packed and shipped in Normal Phase solvents
Suitable for both Normal Phase and HILIC chromatographic modes
Wide pore design improves access for larger molecules and complex carbohydrates
Excellent retention of sugars, carbohydrates, and organic acids
Extended column length maximizes resolution and peak capacity
Ideal for semi-preparative purification and analytical-to-prep method scale-up
Requires an HPLC system capable of supporting semi-preparative flow rates of approximately 8–15 mL/min
This is a Special Order Item